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ocds-b5fd17-f0a5cd0e-71a2-47f9-a409-e20abaae72f9Published facts
c1725ab5-9241-4833-b1ea-aa636dc877f6-779501Royal Botanic Gardens, Kew wishes to appoint a supplier to individually sequence the whole genomes of up to 300 European ash trees (Fraxinus excelsior) at a minimum of 15x whole genome coverage via a long-read technology. Fraxinus excelsior is a diploid and has a 1C genome size of 880 Mbp. In addition to the 300 individual ash trees, we wish to sequence a minimum of six technical replicates. Therefore, the total number of samples we wish to sequence is 306. RBG Kew intends to supply total genomic DNA for each sample with the following metrics: minimum of 10ug DNA; minimum concentration of 30ng/ml (as measured with a Quantus flurometer dsDNA assay); at least 50% of the sample in fragments with a minimum length of 40kb; minimum OD260/280 of 1.8; minimum OD260/230 of 1.8; minimum DIN of 7.
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c1725ab5-9241-4833-b1ea-aa636dc877f6-779501Awards and suppliers
Buyer: Royal Botanic Gardens, KewSupplier: Novogene (UK) Company LTD
ocds-b5fd17-f0a5cd0e-71a2-47f9-a409-e20abaae72f9-1End dates, recurrence wording and links are shown as they appear in public notices. They do not create a new opportunity. If no later linked record appears here, that means Bidwake did not find one in the records it has loaded; it does not prove that none exists.